England captain Harry Kane has told those behind the racist abuse of team-mates Marcus Rashford, Jadon Sancho and Bukayo Saka: "You're not an England fan and we don't want you."
All three players missed penalties in the 3-2 shootout loss to Italy in Sunday's Euro 2020 final.
They were targeted on social media after the game.
"They deserve support and backing, not the vile racist abuse they've had since last night," Kane said.
"Three lads who were brilliant all summer had the courage to step up and take a pen when the stakes were high," the Tottenham striker added on Twitter.external-link
"If you abuse anyone on social media you're not an England fan and we don't want you."
Defender Tyrone Mings also took to Twitter to talk of his pride in the team reaching the final but addedexternal-link: "Waking up today and seeing my brothers being racially abused for being brave enough to put themselves in a position to help this country, is something that sickens, but doesn't surprise me."
He went on to criticise Home Secretary Priti Patel who last month described players taking the knee against racism as "gesture politics".
Earlier, manager Gareth Southgate said the racist abuse issued after the defeat was "unforgivable".
Prime Minister Boris Johnson and the Football Association also condemned it.
The Metropolitan Policeexternal-link is investigating the abuse and said "it will not be tolerated", while the UK Football Policing Unit (UKFPU) has also launched an investigation.
Chief Constable Mark Roberts, the National Police Chiefs' Council Football Policing Lead, said police were working with Facebook, Instagram and Twitter "and enquiries are already being progressed".
"It's just not what we stand for," said Southgate.
"We have been a beacon of light in bringing people together, in people being able to relate to the national team, and the national team stands for everybody and so that togetherness has to continue.
"We have shown the power our country has when it does come together and has that energy and positivity together.
"It's my decision who takes the penalties, it's not a case of players not volunteering or more experienced players backing out."
England and Borussia Dortmund midfielder Jude Bellingham tweeted a picture of the three players wearing crowns and wrote: "We win together and we lose together. So proud to have team-mates with such top character. As for the racism, hurtful but not surprising. Will never get bored of saying that more needs to be done. Educate and control the platforms."
Jadon Sancho and Marcus Rashford missed penalties in England's shootout defeatEuropean football's governing body Uefa condemned the "disgusting racist abuse", adding: "We stand by the players and the FA's call for the strongest possible punishments."
On Monday, League Two side Leyton Orient said they had banned a fan for life in connection with the abuse.
"The supporter in question's actions on Twitter were alerted to the club late last night, and action has been taken swiftly to issue a banning order," the club said.
Meanwhile, Premier League side Wolves said they had passed details to police after being alerted to racist abuse from one of their supporters.
And a parish councillor in Shropshire has resigned after racist posts appeared on his personal Facebook page in the wake of England's defeat.
Paul Bradbury, a member of Pontesbury Parish Council, said his account had been hacked.
West Mercia Police said it had arrested a man in his 60s from Minsterley, Shropshire, on suspicion of inciting racial hatred and he remained in police custody.
England had reached their first final in a major tournament since winning the World Cup in 1966 and, despite taking the lead against Italy, drew 1-1 after extra time before a penalty shootout.
"This England team deserve to be lauded as heroes, not racially abused on social media," said the prime minister.external-link
He later started a Downing Street briefing by praising the players, adding: "They brought joy to this country and to those who have been directing racist abuse I say 'shame on you and I hope you will crawl back under the rock from which you emerged'."
England's players have taken a knee before games at the Euros to highlight the fight against racial inequality.
On the day the tournament started on 11 June, the prime minister did not condemn fans who jeered when England players took the knee during two warm-up games.
Instead, Johnson said he wanted to see fans "getting behind the team to cheer them on" - and was then accused of not having "the guts to call it out" by Labour leader Sir Keir Starmer.
We implore government to act quickly - FA
The FA said it was "appalled" by the "online racism" directed at the three players following the defeat at Wembley.
It added: "We could not be clearer that anyone behind such disgusting behaviour is not welcome in following the team.
"We will do all we can to support the players affected while urging the toughest punishments possible for anyone responsible.
"We will continue to do everything we can to stamp discrimination out of the game, but we implore government to act quickly and bring in the appropriate legislation so this abuse has real life consequences.
"Social media companies need to step up and take accountability and action to ban abusers from their platforms, gather evidence that can lead to prosecution and support making their platforms free from this type of abhorrent abuse."
In February, the government threatened social media companies with "large fines" if they failed to tackle abuse on their platforms.
"It's unbelievable that we are still talking about it," FA chief executive Mark Bullingham told BBC Sport. "We've been so clear, these social media companies need to act. They need to stamp it out and they can do that. "We are reiterating a call for the government to bring in the Online Harms Bill as soon as they can. We don't think it's acceptable in any walk of life for this racist abuse to happen and we want it to stop."
Rashford highlighted racial abuse he received on social media in May after losing the Europa League final with Manchester United.
And last year Sancho was among sport stars protesting against racism following the murder of George Floyd by a police officer in Minneapolis.
Social media companies have been criticised for a perceived lack of action on racist abuse on their platforms, and in April Instagram announced a tool to enable users to automatically filter out abusive messages from those they do not follow.
Following numerous instances of online abuse, a number of clubs, players, athletes and sporting bodies took part in a four-day boycott of social media in April to encourage companies to take a stronger stance against racist and sexist abuse.
On the back of the abuse suffered by England's players after the Euro 2020 final, Facebook said it had recently announced tougher measures on its Instagram platform, including permanently deleting accounts that repeatedly send abusive direct messages.
"No one should have to experience racist abuse anywhere, and we don't want it on Instagram," said a Facebook spokesperson.
"We quickly removed comments and accounts directing abuse at England's footballers last night and we'll continue to take action against those that break our rules.
"In addition to our work to remove this content, we encourage all players to turn on Hidden Words, a tool which means no one has to see abuse in their comments or DMs.
"No one thing will fix this challenge overnight, but we're committed to keeping our community safe from abuse."
Twitter said it had removed more than 1,000 posts over the past 24 hours and suspended a number of accounts for violating its rules.
Southgate said his team would "heal together as a team now" and that "we're there for them [Sancho, Rashford and Saka] and I know that 99% of the public will be as well".
He added: "Bukayo in particular has been an absolute star in this tournament, incredible maturity and the way he has played has brought a smile to so many people's faces.
"He's become such a popular member of the group and I know he has got everybody's support."
The Professional Footballers' Association joined the condemnation, saying those responsible "represent the worst of us", and that the response from social media companies was "insufficient".
"Racist abuse causes trauma. It will impact the targeted players, their team-mates, and we know it will also affect their peers," the PFA said.
"It causes hurt to all the other fans who view online hate, and it will inevitably live with the next aspiring generation of young players."
Ugo Monye shares his experience of 'disgraceful' fans' behaviour at WembleyArsenal proud of Saka - and other reaction
Former England striker and BBC pundit Alan Shearer said he hoped "social media companies expose these individuals" who post online abuse.
"There are so many positives for social media but also so many negatives," Shearer told BBC Breakfast.
"The positives are that it allows so many of these players and squad to show their personality on the pitch and, most importantly, off the pitch.
"But on the negative side, what on earth are people thinking about - when these guys have been brave enough to take a penalty - to go online and abuse these players?
"What is wrong with some people? It is just so sad and ridiculous. I hope the social media companies expose these individuals. Absolutely disgusting."
Saka, who had his penalty saved by Italy keeper Gianluigi Donnarumma, was consoled by his team-mates and England manager Southgate following his spot-kick.
The 19-year-old has been at Arsenal since he was seven and the Gunners said they were "proud" of the winger's exploits at Euro 2020 and told him to "hold your head high".
"Last night we witnessed the leadership and character we've always known and loved in Bukayo," said an Arsenal statement.
"However, this feeling of pride quickly turned to sorrow at the racist comments our young player was subjected to on his social media platforms after the final whistle.
"Once again, we are sad to have to say we condemn the racism of a number of black players.
"This cannot continue and the social media platforms and authorities must act to ensure this disgusting abuse to which our players are subjected on a daily basis stops now.
"We have processes in place internally at Arsenal to ensure our players are supported both emotionally and practically on this issue but sadly there is only so much we can do."
Seven-time Formula 1 world champion Lewis Hamilton wrote on Instagram it "shows how much work still needs to be done" to tackle the problem of online racist abuse.
Former England defender Gary Neville criticised the stance of the prime minister and feared the worst when Sancho, Rashford and Saka missed their penalties.
"I wasn't surprised in the slightest that I woke up to those headlines," Neville told Sky News.
"I expected it the minute that the three players that missed, missed."
Analysis
BBC technology correspondent Rory Cellan-Jones
This is the biggest test yet for the social media giants in their attempts to crack down on racial abuse of footballers - and so far it's not going well.
Such is the volume of abusive comments that the companies rely increasingly on automated systems to weed it out. But these have severe limitations. On example - an orangutan emoji was used in an Instagram comment about one England footballer. But the user who reported it got a message saying "our technology has found that this comment doesn't go against our Community Guidelines."
The message from Instagram went on to admit "our technology isn't perfect" - and made it clear that the human moderation team hadn't been able to review the decision because of the volume of reports.
There will be pressure on the companies to make it impossible to use certain words or emojis - but they will point to criticism from others who say they should not be policing speech that may be distasteful but is not illegal.
