England midfielder Keira Walsh was taken off the pitch on a stretcher in the 38th minute during the World Cup match against Denmark.
The Barcelona player, 26, appeared to hurt her knee and looked in distress before signalling to the bench that she could not carry on.
She was comforted by team-mates and appeared to mouth: "I've done my knee."
England are already without captain Leah Williamson and forward Beth Mead with long-term knee injuries.
Walsh left the pitch when the Lionesses were leading 1-0 against Denmark in Sydney in their second Group D match at the World Cup.
They play China in their final group game on Tuesday in Adelaide.
