Federer (20) and Nadal (21) have won a total of 41 major titlesRoger Federer's final match will see him play alongside old rival Rafael Nadal in the Laver Cup doubles on Friday.
The pair will represent Team Europe against Team World's Jack Sock and Frances Tiafoe at London's 02 Arena.
Before that match, Andy Murray will start the evening session in singles against Alex de Minaur at 19:00 BST.
Federer, a 20-time Grand Slam winner, said last week he would retire at the team event, which starts on Friday.
The 41-year-old Swiss had said it would be the "most beautiful thing" to play alongside Spaniard Nadal, 36, who has 22 major titles.
Federer has struggled with a knee problem and does not feel able to play singles.
His last competitive match was a defeat by Hubert Hurkacz in last year's Wimbledon quarter-finals.
Europe take on a world team in the three-day Laver Cup, where Italian Matteo Berrettini will take Federer's place over the weekend.
More to follow
