World number one Jon Rahm and Europe's Ryder Cup record points scorer Sergio Garcia will take on American favourites Jordan Spieth and Justin Thomas in Friday's opening match in Wisconsin.
Garcia has amassed 25½ points, the same as the entire US team combined, although Spieth and Thomas won three of their four matches together in Paris.
Europe captain Padraig Harrington has opted for experience in the last of the four matches, putting Rory McIlroy and Ian Poulter together. They face rookies Patrick Cantlay and Xander Schauffele.
Cantlay won the PGA Tour's season-ending Tour Championship earlier this month and was named player of the year by his fellow professionals, while Schauffele won the Olympic title in Tokyo in August.
The 43rd Ryder Cup at Whistling Straits on the shore of Lake Michigan will open with four foursomes (alternate shot) matches, with four fourball matches played in Friday's second session. The pairings for those matches will be announced towards the end of the foursomes.
American captain Steve Stricker is using four of his six debutants in the opening session, with Open champion Collin Morikawa partnering world number two Dustin Johnson.
Europe have three debutants in their 12-strong side but only Viktor Hovland is being used in the morning session, and he will play with England's Paul Casey against Morikawa and Johnson..
Four-time major champion Brooks Koepka, who said in the run-up to this event that he finds it tough to play at a Ryder Cup because the event takes him away from his usual routine, is playing with another debutant in Daniel Berger against English pair Lee Westwood and Matt Fitzpatrick.
More to follow.