Jason Kenny became the first Briton to win seven Olympic gold medals with a spectacular defence of his men's keirin title - but wife Laura's reign as women's omnium champion has ended.
Laura, GB's most successful female athlete, recovered from a crash in her first event to finish sixth in her bid for a third successive omnium title.
Jason stunned the men's keirin field as he sprinted clear with three laps remaining to claim victory.
It is a ninth Olympic medal for him.
The 33-year-old won silver in the team sprint to become GB's most decorated Olympian earlier this week at the Izu Velodrome.
His tally of seven golds is one more than former team-mate Chris Hoy.
Jason Kenny is the most successful British Olympian, while Laura Kenny is Britain's most successful female OlympianJason caught his five rivals by surprise with a long-range attack and held on to finish 0.763 seconds clear of Malaysia's Azizulhasni Awang, with the Netherlands' reigning world champion Harrie Lavreysen taking bronze.
Lavreysen made it three medals in Tokyo but had been aiming to emulate Kenny's achievement of five years ago by winning all three men's sprint events.
Kenny progressed from a semi-final that also included compatriot and individual sprint bronze medallist Jack Carlin, but the 24-year-old failed to qualify for the final after finishing fourth.
Contesting the race to decide the minor positions, Carlin finished second to claim an eighth-placed overall finish.
In the omnium, Laura arrived at the fourth and final event - the points race - down in ninth position and 38 points adrift of top place after a fall in the scratch event.
Brought down in a pile-up on the penultimate lap of the competition's first of four disciplines, Kenny then came out on top in the tempo race before finishing a disappointing 13th in the elimination race - widely considered her specialist event.
The Briton - along with nine others - received 16 points as the officials decided to award the fallen riders the next available number of points in the opening scratch race, placing Kenny 24 points behind the early lead.
The five-time Olympic gold medallist, who became the first British woman to win gold at three Olympic Games when she and Katie Archibald won the madison on Friday, recovered to fifth at the halfway stage after winning the tempo.
However, she was left with too much to do in the final points race - despite taking maximum points in the final sprint - as American Jennifer Valente took gold from Japan's Yumi Kajihara and Dutch rider Kirsten Wild.
Between them, Jason (nine) and Laura Kenny (six) have won 15 Olympic medals